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recombinant human activin a  (R&D Systems)


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    Structured Review

    R&D Systems recombinant human activin a
    Recombinant Human Activin A, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+activin+a+antibody/Recombinant+Human+Activin+AB+Protein/bio_rxiv__64898__2026__04__14__718527-142-7-11
    Average 94 stars, based on 58 article reviews
    recombinant human activin a - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Inhibition:

    Article Title: Activin A secretion by muscle-repairing macrophages induces heterotopic ossification in mice
    Article Snippet: .. For the inhibition of activin A activity, 1 mg/mL anti–activin A antibody (catalog AF338; R&D Systems) was added to the osteogenic differentiation medium. ..

    Activity Assay:

    Article Title: Activin A secretion by muscle-repairing macrophages induces heterotopic ossification in mice
    Article Snippet: .. For the inhibition of activin A activity, 1 mg/mL anti–activin A antibody (catalog AF338; R&D Systems) was added to the osteogenic differentiation medium. ..

    Recombinant:

    Article Title: Activin A Sustains the Metastatic Phenotype of Tumor-Associated Macrophages and Is a Prognostic Marker in Human Cutaneous Melanoma.
    Article Snippet: .. When specified, recombinant human activin A (Immunotools), blocking anti-activin A antibody (1 mg/ ml; polyclonal; R&D Systems), goat IgG isotype‒control antibody (1 mg/ml; R&D Systems), ALK4/5/7 inhibitor (SB-431542, 25 mM; 30 minutes pretreatment, Sigma-Aldrich), COX-2 inhibitor (Celecoxib, 10 mM; Sigma-Aldrich), or 15-hydroxy prostaglandin dehydrogenase inhibitor (SW033291, 1 mM; MedChemExpress, Monmouth Junction, NJ), were added every 24 hours. .. Lymphocytes negatively isolated from PBMCs with CD14 microbeads were labeled with 1 mM carboxyfluorescein diacetate succinimidyl www.jidonline.org 659 660 ester and cultured (105 cells per 200 ml RPMI medium supplemented with 10% fetal calf serum and 50 U/ml IL-2) with Dynabeads Human T-Activator CD3/CD28 (Gibco) at a cell-to-bead ratio of 1:1.

    Blocking Assay:

    Article Title: Activin A Sustains the Metastatic Phenotype of Tumor-Associated Macrophages and Is a Prognostic Marker in Human Cutaneous Melanoma.
    Article Snippet: .. When specified, recombinant human activin A (Immunotools), blocking anti-activin A antibody (1 mg/ ml; polyclonal; R&D Systems), goat IgG isotype‒control antibody (1 mg/ml; R&D Systems), ALK4/5/7 inhibitor (SB-431542, 25 mM; 30 minutes pretreatment, Sigma-Aldrich), COX-2 inhibitor (Celecoxib, 10 mM; Sigma-Aldrich), or 15-hydroxy prostaglandin dehydrogenase inhibitor (SW033291, 1 mM; MedChemExpress, Monmouth Junction, NJ), were added every 24 hours. .. Lymphocytes negatively isolated from PBMCs with CD14 microbeads were labeled with 1 mM carboxyfluorescein diacetate succinimidyl www.jidonline.org 659 660 ester and cultured (105 cells per 200 ml RPMI medium supplemented with 10% fetal calf serum and 50 U/ml IL-2) with Dynabeads Human T-Activator CD3/CD28 (Gibco) at a cell-to-bead ratio of 1:1.

    Immunohistochemical staining:

    Article Title: Short-term overloading exercise attenuates articular chondrocyte features partly via synovium-cartilage interactions mediated by inhibin subunit beta A.
    Article Snippet: Safranin O staining was performed according to standard protocols, and images of all stained slides were captured using a NanoZoomer S60 (Hamamatsu Photonics, Shizuoka, Japan). .. For immunohistochemical staining, the tissue sections were incubated overnight at 4 °C with anti-activin A antibody (5 μg/mL, AF-338, R&D Systems, USA). .. After incubation with horseradish peroxidase-conjugated secondary antibodies for 30 min at room temperature, positive signals were visualized using Alexa Fluor (B40958, Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Short-term overloading exercise attenuates articular chondrocyte features partly via synovium–cartilage interactions mediated by inhibin subunit beta A
    Article Snippet: Safranin O staining was performed according to standard protocols, and images of all stained slides were captured using a NanoZoomer S60 (Hamamatsu Photonics, Shizuoka, Japan). .. For immunohistochemical staining, the tissue sections were incubated overnight at 4 °C with anti-activin A antibody (5 μg/mL, AF-338, R&D Systems, USA). .. After incubation with horseradish peroxidase-conjugated secondary antibodies for 30 min at room temperature, positive signals were visualized using Alexa Fluor (B40958, Thermo Fisher Scientific, Waltham, MA, USA).

    Staining:

    Article Title: Short-term overloading exercise attenuates articular chondrocyte features partly via synovium-cartilage interactions mediated by inhibin subunit beta A.
    Article Snippet: Safranin O staining was performed according to standard protocols, and images of all stained slides were captured using a NanoZoomer S60 (Hamamatsu Photonics, Shizuoka, Japan). .. For immunohistochemical staining, the tissue sections were incubated overnight at 4 °C with anti-activin A antibody (5 μg/mL, AF-338, R&D Systems, USA). .. After incubation with horseradish peroxidase-conjugated secondary antibodies for 30 min at room temperature, positive signals were visualized using Alexa Fluor (B40958, Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Short-term overloading exercise attenuates articular chondrocyte features partly via synovium–cartilage interactions mediated by inhibin subunit beta A
    Article Snippet: Safranin O staining was performed according to standard protocols, and images of all stained slides were captured using a NanoZoomer S60 (Hamamatsu Photonics, Shizuoka, Japan). .. For immunohistochemical staining, the tissue sections were incubated overnight at 4 °C with anti-activin A antibody (5 μg/mL, AF-338, R&D Systems, USA). .. After incubation with horseradish peroxidase-conjugated secondary antibodies for 30 min at room temperature, positive signals were visualized using Alexa Fluor (B40958, Thermo Fisher Scientific, Waltham, MA, USA).

    Incubation:

    Article Title: Short-term overloading exercise attenuates articular chondrocyte features partly via synovium-cartilage interactions mediated by inhibin subunit beta A.
    Article Snippet: Safranin O staining was performed according to standard protocols, and images of all stained slides were captured using a NanoZoomer S60 (Hamamatsu Photonics, Shizuoka, Japan). .. For immunohistochemical staining, the tissue sections were incubated overnight at 4 °C with anti-activin A antibody (5 μg/mL, AF-338, R&D Systems, USA). .. After incubation with horseradish peroxidase-conjugated secondary antibodies for 30 min at room temperature, positive signals were visualized using Alexa Fluor (B40958, Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Short-term overloading exercise attenuates articular chondrocyte features partly via synovium–cartilage interactions mediated by inhibin subunit beta A
    Article Snippet: Safranin O staining was performed according to standard protocols, and images of all stained slides were captured using a NanoZoomer S60 (Hamamatsu Photonics, Shizuoka, Japan). .. For immunohistochemical staining, the tissue sections were incubated overnight at 4 °C with anti-activin A antibody (5 μg/mL, AF-338, R&D Systems, USA). .. After incubation with horseradish peroxidase-conjugated secondary antibodies for 30 min at room temperature, positive signals were visualized using Alexa Fluor (B40958, Thermo Fisher Scientific, Waltham, MA, USA).

    Control:

    Article Title: Integrin-TGFβ axis induces partial EMT in basal-like cells to lead collective invasion
    Article Snippet: .. The following antibodies were used: rabbit anti-human Vimentin (Cat#ab92547, Abcam), chicken anti-human Vimentin (Cat#PA1-16759, Invitrogen) rabbit anti-human Keratin 14 (Cat# 905301, Biolegend), rat anti-mouse Keratin 8 (Cat# 531826, DSHB), rabbit anti-rat Collagen I cleavage site (Col 3⁄4, Cat#0217-025, immunoGlobe), rabbit anti-human integrin α2 (Cat#ab181548, Abcam), rabbit anti-human Inhba (Cat#10651-1-AP, Proteintech), Mouse IgG1 isotype control (MAB002, R&D Systems), anti-Activin A antibody (Cat#AF338, R&D Systems). .. The following secondary antibodies were used: Alexa fluor-488/568/647-conjugated goat anti-mouse, -rat, or -rabbit antibody (Invitrogen).

    other:

    Article Title: Stepwise release of Activin-A from its inhibitory prodomain is modulated by cysteines and requires furin coexpression to promote melanoma growth
    Article Snippet: For plasmon resonance measurements, gold nanohole array plasmonic chips fabricated at EPFL , were functionalized by precoating with anti-Activin-A antibody (BAM050, R&D Systems), or with AIIB-Fc (339-RBB-100).



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